Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants

Y. Matsushita, K. Yoshioka, T. Shigyo, H. Takahashi, H. Nyunoya

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The 3a protein of Cucumber mosaic virus is essential for the cell-to-cell movement of the viral RNA through plasmodesmata. We have introduced an epitope peptide before the stop codon of the 3a protein and cloned the tagged ORF into a binary vector for Agrobacterium-mediated transformation. The established transgenic tobacco lines produced the 3a protein, which was specifically detected with anti-3a and anti-epitope antisera. Metabolic labeling and subsequent immunoprecipitation revealed that [32P]-orthophosphate was incorporated into the 3a protein. The phosphoamino acid analysis indicated that the 3a protein contained phosphoserine but not phosphothreonine or phosphotyrosine. This is the first demonstration of the 3a protein phosphorylation in planta.

Original languageEnglish
Pages (from-to)231-234
Number of pages4
JournalVirus Genes
Volume24
Issue number3
DOIs
Publication statusPublished - 2002 Jun 20

Keywords

  • Cucumber mosaic virus
  • Movement protein
  • Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Fingerprint Dive into the research topics of 'Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants'. Together they form a unique fingerprint.

  • Cite this