Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants

Y. Matsushita, K. Yoshioka, T. Shigyo, H. Takahashi, H. Nyunoya

    Research output: Contribution to journalArticlepeer-review

    32 Citations (Scopus)

    Abstract

    The 3a protein of Cucumber mosaic virus is essential for the cell-to-cell movement of the viral RNA through plasmodesmata. We have introduced an epitope peptide before the stop codon of the 3a protein and cloned the tagged ORF into a binary vector for Agrobacterium-mediated transformation. The established transgenic tobacco lines produced the 3a protein, which was specifically detected with anti-3a and anti-epitope antisera. Metabolic labeling and subsequent immunoprecipitation revealed that [32P]-orthophosphate was incorporated into the 3a protein. The phosphoamino acid analysis indicated that the 3a protein contained phosphoserine but not phosphothreonine or phosphotyrosine. This is the first demonstration of the 3a protein phosphorylation in planta.

    Original languageEnglish
    Pages (from-to)231-234
    Number of pages4
    JournalVirus Genes
    Volume24
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Cucumber mosaic virus
    • Movement protein
    • Phosphorylation

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Virology

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