Phosphorylation of farnesol by a cell-free system from botryococcus braunii

Hitoshi Inoue, Tatsumi Korenaga, Hiroshi Sagami, Tanetoshi Koyama, Kyozo Ogura

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Farnesol was incorporated into squalene as well as botryococcenes when the alcohol was fed to the culture of Botryococcus braunii B race strain. In in vitro experiments with a 10,000 × g supernatant of cell homogenate, squalene was synthesized from farnesyl diphosphate in the presence of NADPH or NADH, but botryococcenes were not synthesized under the same conditions. A 100,000 × g pelet fraction was able to phosphorylate farnesol to give its mono- and diphosphate esters in a CTP dependent manner.

Original languageEnglish
Pages (from-to)1036-1041
Number of pages6
JournalBiochemical and biophysical research communications
Volume200
Issue number2
DOIs
Publication statusPublished - 1994 Apr 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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