Phosphorylation and activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase by a brain Ca2+, calmodulin-dependent protein kinase

Kohji Fukunaga; Hideyuki Yamamoto; Etsuro Tanaka; Takafumi Iwasa; Eishichi Miyamoto

doi:10.1016/0024-3205(84)90242-X

Phosphorylation and activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase by a brain Ca²⁺, calmodulin-dependent protein kinase

Kohji Fukunaga, Hideyuki Yamamoto, Etsuro Tanaka, Takafumi Iwasa, Eishichi Miyamoto

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A Ca²⁺, calmodulin-dependent protein kinase from rat brain with a MW of 640,000 phosphorylated calmodulin-sensitive phosphodiesterase from the brain cytosol. The Km of the enzyme for the phosphodiesterase was 5.0 μM and the Vmax was 212 nmol/mg/min. The amount of phosphate incorporated into the phosphodiesterase was 0.7 mol/mol subunit. Phosphorylation of the phosphodiesterase enhanced the enzyme activity by about 20% for hydrolysis of a higher concentration of cyclic AMP.

Original language	English
Pages (from-to)	493-499
Number of pages	7
Journal	Life Sciences
Volume	35
Issue number	5
DOIs	https://doi.org/10.1016/0024-3205(84)90242-X
Publication status	Published - 1984 Jul 30
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(84)90242-X

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abstract = "A Ca2+, calmodulin-dependent protein kinase from rat brain with a MW of 640,000 phosphorylated calmodulin-sensitive phosphodiesterase from the brain cytosol. The Km of the enzyme for the phosphodiesterase was 5.0 μM and the Vmax was 212 nmol/mg/min. The amount of phosphate incorporated into the phosphodiesterase was 0.7 mol/mol subunit. Phosphorylation of the phosphodiesterase enhanced the enzyme activity by about 20% for hydrolysis of a higher concentration of cyclic AMP.",

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AU - Fukunaga, Kohji

AU - Yamamoto, Hideyuki

AU - Tanaka, Etsuro

AU - Iwasa, Takafumi

AU - Miyamoto, Eishichi

PY - 1984/7/30

Y1 - 1984/7/30

N2 - A Ca2+, calmodulin-dependent protein kinase from rat brain with a MW of 640,000 phosphorylated calmodulin-sensitive phosphodiesterase from the brain cytosol. The Km of the enzyme for the phosphodiesterase was 5.0 μM and the Vmax was 212 nmol/mg/min. The amount of phosphate incorporated into the phosphodiesterase was 0.7 mol/mol subunit. Phosphorylation of the phosphodiesterase enhanced the enzyme activity by about 20% for hydrolysis of a higher concentration of cyclic AMP.

AB - A Ca2+, calmodulin-dependent protein kinase from rat brain with a MW of 640,000 phosphorylated calmodulin-sensitive phosphodiesterase from the brain cytosol. The Km of the enzyme for the phosphodiesterase was 5.0 μM and the Vmax was 212 nmol/mg/min. The amount of phosphate incorporated into the phosphodiesterase was 0.7 mol/mol subunit. Phosphorylation of the phosphodiesterase enhanced the enzyme activity by about 20% for hydrolysis of a higher concentration of cyclic AMP.

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Phosphorylation and activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase by a brain Ca²⁺, calmodulin-dependent protein kinase

Abstract

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