Phosphorylated tau in human cerebrospinal fluid is a diagnostic marker for Alzheimer's disease

Koichi Ishiguro, Hideto Ohno, Hiroyuki Arai, Haruyasu Yamaguchi, Katsuya Urakami, Jung Mi Park, Kazuki Sato, Hideki Kohno, Kazutomo Imahori

Research output: Contribution to journalArticlepeer-review

163 Citations (Scopus)


Microtubule-associated protein tau in cerebrospinal fluid (CSF) has been proposed as a diagnostic marker for Alzheimer's disease (AD), but there is overlap between AD patients and non-AD controls. To improve the diagnostic accuracy, we measured phosphorylated tau in CSF, because phosphorylated tau accumulates as pathological paired helical filaments in neurons of the AD brain. Immunoblot showed that CSF contained a 32 kDa N-terminal fragment of tau that was partially phosphorylated on Ser199, Thr231 and Ser235. A sandwich enzyme immunoassay revealed that phosphorylated CSF-tau levels were significantly higher in AD patients than those in non-AD controls. Discrimination between the two groups was clearer in phosphorylated CSF-tau than in total CSF-tau. The data indicate that elevated phosphorylated CSF- tau level is a more specific diagnostic marker for AD.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
JournalNeuroscience Letters
Issue number2
Publication statusPublished - 1999 Jul 30
Externally publishedYes


  • Alzheimer's disease
  • Cerebrospinal fluid
  • Diagnosis
  • Enzyme immunoassay
  • Phosphorylation
  • Tau

ASJC Scopus subject areas

  • Neuroscience(all)


Dive into the research topics of 'Phosphorylated tau in human cerebrospinal fluid is a diagnostic marker for Alzheimer's disease'. Together they form a unique fingerprint.

Cite this