Phosphorylated tau in human cerebrospinal fluid is a diagnostic marker for Alzheimer's disease

Koichi Ishiguro, Hideto Ohno, Hiroyuki Arai, Haruyasu Yamaguchi, Katsuya Urakami, Jung Mi Park, Kazuki Sato, Hideki Kohno, Kazutomo Imahori

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Microtubule-associated protein tau in cerebrospinal fluid (CSF) has been proposed as a diagnostic marker for Alzheimer's disease (AD), but there is overlap between AD patients and non-AD controls. To improve the diagnostic accuracy, we measured phosphorylated tau in CSF, because phosphorylated tau accumulates as pathological paired helical filaments in neurons of the AD brain. Immunoblot showed that CSF contained a 32 kDa N-terminal fragment of tau that was partially phosphorylated on Ser199, Thr231 and Ser235. A sandwich enzyme immunoassay revealed that phosphorylated CSF-tau levels were significantly higher in AD patients than those in non-AD controls. Discrimination between the two groups was clearer in phosphorylated CSF-tau than in total CSF-tau. The data indicate that elevated phosphorylated CSF- tau level is a more specific diagnostic marker for AD.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
JournalNeuroscience Letters
Volume270
Issue number2
DOIs
Publication statusPublished - 1999 Jul 30

Keywords

  • Alzheimer's disease
  • Cerebrospinal fluid
  • Diagnosis
  • Enzyme immunoassay
  • Phosphorylation
  • Tau

ASJC Scopus subject areas

  • Neuroscience(all)

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    Ishiguro, K., Ohno, H., Arai, H., Yamaguchi, H., Urakami, K., Park, J. M., Sato, K., Kohno, H., & Imahori, K. (1999). Phosphorylated tau in human cerebrospinal fluid is a diagnostic marker for Alzheimer's disease. Neuroscience Letters, 270(2), 91-94. https://doi.org/10.1016/S0304-3940(99)00476-0