TY - JOUR
T1 - Phosphorothiolate ribozyme against the conserved sequence in V3 loop of HIV-1
AU - Zhang, Xiaoyan
AU - Yamada, Ryo
AU - Shimayama, Takashi
AU - Imada, Kazunori
AU - Uchiyama, Takashi
AU - Hattori, Toshio
N1 - Funding Information:
This work was supported by a grant-in-aid from the Ministry for Education, Science, and Culture of Japan (07557078). We are grateful to Drs. J Sodroski at Harvard University and C. Weiss at FDA for supplying pSVIIIenv3-2 and CHO-WT through NIH AIDS Research and Reference Reagent Program, respectively. We are indebted to Dr. S. Toyama for confocal microscopy and for helpful discussion.
PY - 1996/12/13
Y1 - 1996/12/13
N2 - Fluorescent-labeled phosphorothiolate DNA-RNA chimeric hammerhead ribozyme (thio-Rz) was synthesized. The ribozyme recognizes relatively conserved sequences in the functionally important region (V3 loop) of envelope protein of HIV-1. The thio-Rz as well as wild-type ribozyme cleaved RNA transcripts (414 base) of V3 region in a dose-dependent manner. After delivery into the cells, both wild-type and thio-Rz were able to be readily detected. The positive signals faded within 12 h in the former case, while they lasted 24 h in the latter case. The inhibitory effect by thio-Rz on cell fusion between envelope-expressing CHO cells and CD4+ cells appeared to be time-dependent, and about 70% of inhibition was seen at 72 h. Possible applications of the ribozyme against HIV-1 infection were discussed.
AB - Fluorescent-labeled phosphorothiolate DNA-RNA chimeric hammerhead ribozyme (thio-Rz) was synthesized. The ribozyme recognizes relatively conserved sequences in the functionally important region (V3 loop) of envelope protein of HIV-1. The thio-Rz as well as wild-type ribozyme cleaved RNA transcripts (414 base) of V3 region in a dose-dependent manner. After delivery into the cells, both wild-type and thio-Rz were able to be readily detected. The positive signals faded within 12 h in the former case, while they lasted 24 h in the latter case. The inhibitory effect by thio-Rz on cell fusion between envelope-expressing CHO cells and CD4+ cells appeared to be time-dependent, and about 70% of inhibition was seen at 72 h. Possible applications of the ribozyme against HIV-1 infection were discussed.
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U2 - 10.1006/bbrc.1996.1827
DO - 10.1006/bbrc.1996.1827
M3 - Article
C2 - 8954921
AN - SCOPUS:17144447773
VL - 229
SP - 466
EP - 471
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -
