Phosphorothiolate ribozyme against the conserved sequence in V3 loop of HIV-1

Xiaoyan Zhang, Ryo Yamada, Takashi Shimayama, Kazunori Imada, Takashi Uchiyama, Toshio Hattori

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Fluorescent-labeled phosphorothiolate DNA-RNA chimeric hammerhead ribozyme (thio-Rz) was synthesized. The ribozyme recognizes relatively conserved sequences in the functionally important region (V3 loop) of envelope protein of HIV-1. The thio-Rz as well as wild-type ribozyme cleaved RNA transcripts (414 base) of V3 region in a dose-dependent manner. After delivery into the cells, both wild-type and thio-Rz were able to be readily detected. The positive signals faded within 12 h in the former case, while they lasted 24 h in the latter case. The inhibitory effect by thio-Rz on cell fusion between envelope-expressing CHO cells and CD4+ cells appeared to be time-dependent, and about 70% of inhibition was seen at 72 h. Possible applications of the ribozyme against HIV-1 infection were discussed.

Original languageEnglish
Pages (from-to)466-471
Number of pages6
JournalBiochemical and biophysical research communications
Volume229
Issue number2
DOIs
Publication statusPublished - 1996 Dec 13

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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