Phospholipid polymer multilayered hydrogels containing cells for cancer drug screening

Kazuhiko Ishihara, Botao Gao, Tomohiro Konno

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Hydrogel multilayers with micrometer-scale in thickness containing living cancer cells were prepared by layer-by-layer assembling with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer solution and poly(vinyl alcohol) (PVA) solution alternatively under biological conditions. It was demonstrated that this tool could be utilized for minimizing barriers to the diffusion of drugs while preserving the 3D context. As a case study, the cytotoxicity effects of paclitaxel (PTX) on human cervical cancer HeLa-Fucci cells were evaluated in vitro. A comparative study was carried out: 2D culture, 3D culture in microgel thin membrane and in macrogel. Time-lapse microscopic analyses showed HeLa-Fucci cells cultured on 2D exhibited a more rapid and robust response to stimulation than cells cultured in microgel thin membrane. Besides, HeLa-Fucci cells cultured under macrogel did not show any response to stimulation. It was speculated that both of the diffusion of PTX and matrix dimensionality could affect drug efficacy and diffusion of drug seemed to play a more important role.

Original languageEnglish
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
DOIs
Publication statusPublished - 2019 Jan 1
Externally publishedYes
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: 2019 Apr 32019 Apr 6

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period19/4/319/4/6

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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