Although a number of phospholipase A1s (PLA1s) have been identified in the recent decade, the physiological functions of PLA1s remain almost elusive. The major reason for this is the poor availability of assay methods. In many studies, radiolabeled phospholipid substrates have been used to measure PLA1 activity. This chapter describes the conventional PLA1 assay using a radiolabeled substrate and a novel PLA1 assay using electrospray ionization mass spectrometry.
|Title of host publication||Lipidomics and Bioactive Lipids|
|Subtitle of host publication||Lipids and Cell Signaling|
|Number of pages||13|
|Publication status||Published - 2007|
|Name||Methods in Enzymology|
ASJC Scopus subject areas
- Molecular Biology