Phospholipase A1 Assays Using a Radiolabeled Substrate and Mass Spectrometry

Rei Morikawa, Masafumi Tsujimoto, Hiroyuki Arai, Junken Aoki

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Although a number of phospholipase A1s (PLA1s) have been identified in the recent decade, the physiological functions of PLA1s remain almost elusive. The major reason for this is the poor availability of assay methods. In many studies, radiolabeled phospholipid substrates have been used to measure PLA1 activity. This chapter describes the conventional PLA1 assay using a radiolabeled substrate and a novel PLA1 assay using electrospray ionization mass spectrometry.

Original languageEnglish
Title of host publicationLipidomics and Bioactive Lipids
Subtitle of host publicationLipids and Cell Signaling
EditorsAlex Brown
Pages1-13
Number of pages13
DOIs
Publication statusPublished - 2007
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume434
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Phospholipase A<sub>1</sub> Assays Using a Radiolabeled Substrate and Mass Spectrometry'. Together they form a unique fingerprint.

Cite this