Phosphate group requirement for mitogenic activation of lymphocytes by an extracellular phosphopolysaccharide from Lactobacillus delbrueckii ssp. bulgaricus

Haruki Kitazawa, Toko Harata, Junko Uemura, Tadao Saito, Tsutomu Kaneko, Takatoshi Itoh

Research output: Contribution to journalArticlepeer-review

150 Citations (Scopus)

Abstract

The mitogenic activity of extracellular polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL 1073R-1 and NCFB2483 was examined in murine lymphocytes. The extracellular polysaccharide from Lactobacillus delbrueckii ssp. bulgaricus OLL 1073R-1 was fractionated into neutral and acidic polysaccharides by anion-exchange chromatography, while that of Lactobacillus delbrueckii ssp. bulgaricus NCFB2483 were all fractionated into neutral polysaccharide(s). The acidic polysaccharide stimulated mitogenic responses of murine splenocytes and Peyer's patches but not of thymocytes. The optimal concentration of the acidic polysaccharide at the highest stimulation was 160 μg/ml. A significant increase of mitogenic activity was initiated at 24 h, and the highest response was obtained after stimulation for 48 h. The acidic polysaccharide purified by high performance liquid chromatography also had substantial mitogenic activity, and the molecular weight was estimated to be 1.2 X 106. The acidic polysaccharide was a phosphopolysaccharide consisting of glucose, galactose and phosphorus. Dephosphorylation by hydrofluoric acid degradation reduced the mitogenic activity in lymphocytes. The phosphopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus 1073R-1 is a potent B-cell-dependent mitogen in which the phosphate group acts as a trigger of the mitogenic induction.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalInternational Journal of Food Microbiology
Volume40
Issue number3
DOIs
Publication statusPublished - 1998 Apr 14

Keywords

  • Lactobacillus delbrueckii ssp. bulgaricus
  • Mitogenic activity
  • Phosphate group
  • Phosphopolysaccharide

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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