Phloem-localizing sulfate transporter, Sultr1;3, mediates re-distribution of sulfur from source to sink organs in arabidopsis

Naoko Yoshimoto, Eri Inoue, Kazuki Saito, Tomoyuki Yamaya, Hideki Takahashi

    Research output: Contribution to journalArticlepeer-review

    140 Citations (Scopus)

    Abstract

    For the effective recycling of nutrients, vascular plants transport pooled inorganic ions and metabolites through the sieve tube. A novel sulfate transporter gene, Sultr1;3, was identified as an essential member contributing to this process for redistribution of sulfur source in Arabidopsis. Sultr1;3 belonged to the family of high-affinity sulfate transporters, and was able to complement the yeast sulfate transporter mutant. The fusion protein of Sultr1;3 and green fluorescent protein was expressed by the Sultr1;3 promoter in transgenic plants, which revealed phloem-specific expression of Sultr1;3 in Arabidopsis. Sultr1;3-green fluorescent protein was found in the sieve element-companion cell complexes of the phloem in cotyledons and roots. Limitation of external sulfate caused accumulation of Sultr1;3 mRNA both in leaves and roots. Movement of 35S-labeled sulfate from cotyledons to the sink organs was restricted in the T-DNA insertion mutant of Sultr1;3. These results provide evidence that Sultr1;3 transporter plays an important role in loading of sulfate to the sieve tube, initiating the source-to-sink translocation of sulfur nutrient in Arabidopsis.

    Original languageEnglish
    Pages (from-to)1511-1517
    Number of pages7
    JournalPlant physiology
    Volume131
    Issue number4
    DOIs
    Publication statusPublished - 2003 Apr 1

    ASJC Scopus subject areas

    • Physiology
    • Genetics
    • Plant Science

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