Phenotype of palmitic acid transport and of signalling in alveolar type II cells from E/H-FABP double-knockout mice: Contribution of caveolin-1 and PPARγ

Florian Guthmann, Christian Schachtrup, Angelika Tölle, Heide Wissel, Bert Binas, Hisatake Kondo, Yuji Owada, Friedrich Spener, Bernd Rüstow

Research output: Contribution to journalReview article

14 Citations (Scopus)

Abstract

Based on the assumption that fatty-acid-binding proteins (FABPs) of the epidermal-type (E-FABP) and heart-type (H-FABP) in murine alveolar type II (TII) cells mediate the synthesis of dipalmitoyl phosphatidylcholine (DPPC), the main surfactant phospholipid, we analysed TII cells isolated from wild-type (wt) and E/H-FABP double-knockout (double-ko) mice. Application of labelled palmitic acid to these cells revealed a drop in uptake, β-oxidation, and incorporation into neutral lipids and total phosphatidylcholine (PC) of TII cells from double-ko mice. Whereas incorporation of labelled palmitic acid into DPPC remained unchanged, degradation studies demonstrated a substantial shift in DPPC synthesis from de novo to reacylation. In addition, increased expression of mRNAs and proteins of caveolin-1 and PPARγ, and an increase of the mRNA encoding fatty acid translocase (FAT) was observed in the double-ko phenotype. As caveolin-1 interacted with PPARγ, we assumed that FAT, caveolin-1, and PPARγ form a signalling chain for fatty acid or drug. Consequently, PPARγ-selective pioglitazone was added to the diet of double-ko mice. We found that further activation of PPARγ could 'heal' the E/H-FABP double-ko effect in these TII cells as transport and utilisation of labelled palmitic acid restored a wt phenocopy. This indicated that E-FABP and/or H-FABP are involved in the mediation of DPPC synthesis in wt TII cells.

Original languageEnglish
Pages (from-to)196-204
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1636
Issue number2-3
DOIs
Publication statusPublished - 2004 Mar 22

Keywords

  • Caveolin-1
  • E-
  • E- and H-FABP
  • E/H-FABP
  • FABP
  • Fatty acid signalling
  • Fatty-acid-binding protein
  • H-
  • Knockout mouse
  • Peroxisome proliferator activated receptor
  • Surfactant
  • double-knockout
  • double-ko
  • epidermal-type
  • fatty-acid-binding protein
  • heart-type

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Phenotype of palmitic acid transport and of signalling in alveolar type II cells from E/H-FABP double-knockout mice: Contribution of caveolin-1 and PPARγ'. Together they form a unique fingerprint.

  • Cite this