Phenobarbital molecularly imprinted polymer selectively binds phenobarbital

Yoshihisa Tomioka, Yoshiki Kudo, Tetsuro Hayashi, Hitoshi Nakamura, Masahiro Niizeki, Takanori Hishinuma, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Molecularly imprinted polymer (MIP) was prepared against phenobarbital using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking monomer. We analyzed the recognition properties of the phenobarbital MIP. In some organic solvents, imprinted polymer showed selective binding to phenobarbital. Two dissociation constants of binding were calculated by Scatchard plot analyses; K(d) values were 1.8, 121.7 μM, and the number of binding sites was 8.3, 92.3 μmol/g MIP in toluene-heptane-acetic acid (25:75:1, v/v), respectively. The relationship between the binding affinity to phenobarbital MIP and the polarity of the solvent system, as well as the structure of the template molecule is also discussed.

Original languageEnglish
Pages (from-to)397-400
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume20
Issue number4
DOIs
Publication statusPublished - 1997 Apr

Keywords

  • antibody mimic
  • molecular imprinting
  • molecular recognition
  • molecularly imprinted polymer (MIP)
  • phenobarbital

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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