Pharmacological characterization of serotonin receptor in the oocyte membrane of bivalve molluscs and its formation during oogenesis

Makoto Osada, Ayumi Nakata, Toshie Matsumoto, Katsuyoshi Mori

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Serotonin (5-hydroxytryptamine, 5-HT) is a major neurotransmitter that triggers the spawning of bivalve molluscs and oocyte germinal vesicle breakdown (GVBD) of oocytes. The present study employed [3H] 5-HT binding assay to characterize a putative receptor on oocyte membranes prepared from scallops and oysters. Results demonstrated a profile of hormone control of 5-HT receptor formation during oocyte growth. The dissociation constants for 5-HT on the surfaces of scallop and oyster oocytes were estimated to be 2.64 μM and 2.01 μM, respectively, and the maximal binding capacity of the oocyte surface was 152 pmol/mg protein in scallops and 529 pmol/mg protein in oysters. The inhibition of [3H] 5-HT bound by various agonists and antagonists provided pharmacological evidence for a 5-HT receptor in each oocyte, a mixed profile of 5-HT1/5-HT2 in scallops and a single profile of 5-HT1 in oyster. Formation of 5-HT receptor on the surface of the scallop oocyte was induced by estradiol-17β(E2), which was suggested as a primary promoter of the effect of E2 on sensitivity to 5-HT, resulting in the induction of spawning. This formation occurred during oocyte growth controlled by E2.

Original languageEnglish
Pages (from-to)124-131
Number of pages8
JournalJournal of Experimental Zoology
Volume281
Issue number2
DOIs
Publication statusPublished - 1998 Jun 1

ASJC Scopus subject areas

  • Animal Science and Zoology

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