TY - JOUR
T1 - pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita
AU - Funamoto, Rintaro
AU - Saito, Katsuharu
AU - Oyaizu, Hiroshi
AU - Aono, Toshihiro
AU - Saito, Masanori
N1 - Funding Information:
We thank Ryo Ohtomo, Tomoko Kojima, and Shotaro Ando (National Agriculture and Food Research Organization) for experimental support. We also thank Yukari Kuga (Hiroshima University) for critical reading of the manuscript. This work was supported in part by the Programme for Promotion of Basic and Applied Research for Innovations in Bio-oriented Industry to KS. This work was also supported in part by the Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) of the Bio-oriented Technology Research Advancement Institution of Japan, and for Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research (B) to MS.
Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.
PY - 2014/1
Y1 - 2014/1
N2 - Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1–6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi.
AB - Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1–6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi.
KW - 6-Carboxyfluorescein diacetate
KW - Arbuscular mycorrhizal symbiosis
KW - Laser scanning confocal microscopy
KW - Tubular vacuole
KW - Vacuolar pH
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U2 - 10.1007/s00572-014-0588-1
DO - 10.1007/s00572-014-0588-1
M3 - Article
C2 - 24838377
AN - SCOPUS:84939877838
VL - 25
SP - 55
EP - 60
JO - Mycorrhiza
JF - Mycorrhiza
SN - 0940-6360
IS - 1
ER -