pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita

Rintaro Funamoto, Katsuharu Saito, Hiroshi Oyaizu, Toshihiro Aono, Masanori Saito

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1–6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalMycorrhiza
Volume25
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • 6-Carboxyfluorescein diacetate
  • Arbuscular mycorrhizal symbiosis
  • Laser scanning confocal microscopy
  • Tubular vacuole
  • Vacuolar pH

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Plant Science

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