Abstract
We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
Original language | English |
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Pages (from-to) | 1952-1963 |
Number of pages | 12 |
Journal | International journal of molecular sciences |
Volume | 14 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2013 Jan |
Externally published | Yes |
Keywords
- Autofluorescence
- FAD
- Fluorescence decay
- Fluorescence lifetime imaging
- Intracellular pH
- Living cell
ASJC Scopus subject areas
- Catalysis
- Molecular Biology
- Spectroscopy
- Computer Science Applications
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry