Abstract
We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
| Original language | English |
|---|---|
| Pages (from-to) | 1952-1963 |
| Number of pages | 12 |
| Journal | International journal of molecular sciences |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 Jan |
Keywords
- Autofluorescence
- FAD
- Fluorescence decay
- Fluorescence lifetime imaging
- Intracellular pH
- Living cell
ASJC Scopus subject areas
- Catalysis
- Molecular Biology
- Spectroscopy
- Computer Science Applications
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry
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Cite this
Islam, M. S., Honma, M., Nakabayashi, T., Kinjo, M., & Ohta, N. (2013). pH dependence of the fluorescence lifetime of FAD in solution and in cells. International journal of molecular sciences, 14(1), 1952-1963. https://doi.org/10.3390/ijms14011952