PH dependence of the fluorescence lifetime of enhanced yellow fluorescent protein in solution and cells

Takakazu Nakabayashi, Shugo Oshita, Ryoya Sumikawa, Fan Sun, Masataka Kinjo, Nobuhiro Ohta

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

pH dependence of the fluorescence decay profile of enhanced yellow fluorescent protein (EYFP) depends on the excitation wavelength. The correlation between the fluorescence lifetime and pH in solution is discussed in terms of the acid-base equilibrium of the chromophore of EYFP. Fluorescence lifetime images of EYFP in HeLa cells have also been measured at various values of intracellular pH. A remarkable pH dependence of the fluorescence lifetime image is observed in pH 4.5-6.0, indicating that pH in a single cell can be evaluated using the fluorescence lifetime image of EYFP especially in the acidic condition.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalJournal of Photochemistry and Photobiology A: Chemistry
Volume235
DOIs
Publication statusPublished - 2012 May 1
Externally publishedYes

Keywords

  • EYFP
  • Fluorescence decay profile
  • Fluorescence lifetime image
  • HeLa cells
  • Intracellular pH

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Physics and Astronomy(all)

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