PGRP-LC and PGRP-LE have essential yet distinct functions in the drosophila immune response to monomeric DAP-type peptidoglycan

Takashi Kaneko, Tamaki Yano, Kamna Aggarwal, Jae Hong Lim, Kazunori Ueda, Yoshiteru Oshima, Camilla Peach, Deniz Erturk-Hasdemir, William E. Goldman, Byung Ha Oh, Shoichiro Kurata, Neal Silverman

Research output: Contribution to journalArticlepeer-review

257 Citations (Scopus)

Abstract

Drosophila rely entirely on an innate immune response to combat microbial infection. Diaminopimelic acid-containing peptidoglycan, produced by Gram-negative bacteria, is recognized by two receptors, PGRP-LC and PGRP-LE, and activates a homolog of transcription factor NF-κB through the Imd signaling pathway. Here we show that full-length PGRP-LE acted as an intracellular receptor for monomeric peptidoglycan, whereas a version of PGRP-LE containing only the PGRP domain functioned extracellularly, like the mammalian CD14 molecule, to enhance PGRP-LC-mediated peptidoglycan recognition on the cell surface. Interaction with the imd signaling protein was not required for PGRP-LC signaling. Instead, PGRP-LC and PGRP-LE signaled through a receptor-interacting protein homotypic interaction motif-like motif. These data demonstrate that like mammals, drosophila use both extracellular and intracellular receptors, which have conserved signaling mechanisms, for innate immune recognition.

Original languageEnglish
Pages (from-to)715-723
Number of pages9
JournalNature Immunology
Volume7
Issue number7
DOIs
Publication statusPublished - 2006 Jul

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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