Persistently adenovirus-infected lymphoid cells express microRNAs derived from the viral VAI and especially VAII RNA

Yuki Furuse, David A. Ornelles, Bryan R. Cullen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Human adenovirus can establish latent infections in lymphoid tissues in vivo and persistent, infections in cultured lymphoid cell lines. During lytic infection, adenovirus expresses microRNAs (miRNAs) derived from the viral non-coding RNAs VAI and, especially, VAII. Here, we demonstrate that persistently adenovirus-infected human BJAB cells also produce adenovirus-derived miRNAs primarily derived from the viral VAII RNA, which contributes ~2.7% of all RNA-induced silencing complex (RISC)-associated RNAs. However, our data indicate that the 5' end of the predominant VAII-derived viral RNA, and hence its seed sequence, differs from what has been previously reported. Our data demonstrate that adenovirus expresses viral miRNAs in chronically infected lymphoid cells and raise the possibility that these may contribute to the maintenance of the latently adenovirus-infected lymphoid cells previously observed in mucosal-associated lymphoid tissues in vivo.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalVirology
Volume447
Issue number1-2
DOIs
Publication statusPublished - 2013 Dec

Keywords

  • Adenovirus
  • MicroRNAs
  • Persistent infection
  • RNA interference

ASJC Scopus subject areas

  • Virology

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