Permeation of redox species through a cell membrane of a single, living algal protoplast studied by microamperometry

Tomoyuki Yasukawa, Isamu Uchida, Tomokazu Matsue

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46 Citations (Scopus)

Abstract

Permeation of several redox species through a cell membrane of a single algal protoplast (radius 100 μm) was investigated by amperometry with a Pt microdisk electrode (disk radius, 6.5 μm) located near the membrane. The redox current observed at the microelectrode decreased as the microelectrode approached the cell membrane since the membrane acted as a barrier for diffusion of redox species from bulk to the microelectrode. Permeability coefficient (P(n)) of the protoplast membrane was determined by the quantitative analysis of the variation of the redox current with micro electrode- membrane distance using digital simulation. The P(m) values for Fe(CN)64-, Fe(CN)63-, Co(phen)32+, ferrocenyl methanol(FMA) and p- hydroquinone(QH2) were ≤ 1.0 x 10-4, ≤ 1.0 x 10-4, 1.0 x 10-3, 5.0 x 10-3 and 2.0 x 10-2 cm/s, respectively. Using these Pm values, the concentration changes inside a model cell and chloroplast were theoretically calculated.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1369
Issue number1
DOIs
Publication statusPublished - 1998 Feb 2

Keywords

  • Algal protoplast
  • Microamperometry
  • Microelectrode
  • Permeability coefficient
  • Single cell

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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