Peritoneal fluids from patients with certain gynecologic tumor contain elevated levels of bioactive lysophospholipase D activity

Akira Tokumura, Tetsuya Kume, Kenji Fukuzawa, Masahiro Tahara, Keiichi Tasaka, Junken Aoki, Hiroyuki Arai, Katsuhiko Yasuda, Hideharu Kanzaki

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


Levels of lysophosphatidic acid (LPA), an important phospholipid mediator, in serum and ascitic fluid from ovarian cancer patients were shown to be higher than those from healthy women and from patients with other type of cancer, respectively. Although LPA in human serum seems mainly to be generated by lysophospholipase D (lysoPLD), the source and pathway for LPA in the ascitic fluid remain still obscure. In this study, we examined whether lysoPLD activity producing bioactive LPA in human peritoneal fluid was significantly elevated under pathological statuses. Lysophospholipase D activity in human peritoneal fluids was measured by quantifying choline released from exogenous lysophosphatidylcholine on their incubation at 37 °C. We also compared the activity of lysoPLD in sera from patients with different gynecologic diseases. We found relatively high lysoPLD activity in peritoneal fluids from patients with ovarian cancer, dermoid cyst or mucinous cystadenoma, whereas there were no significant differences in the serum lysoPLD activity among clinical groups and healthy subjects. The lysoPLD in the peritoneal fluid was found to have similar substrate specificity and metal ion requirement to those of serum lysoPLD, that has been identified as autotaxin, a tumor cell-motility stimulating protein. Our results suggest that increased lysoPLD activity in peritoneal fluid from patients with certain gynecologic tumors might be relevant to its potential of tumor progression.

Original languageEnglish
Pages (from-to)1641-1649
Number of pages9
JournalLife Sciences
Issue number18
Publication statusPublished - 2007 Apr 10
Externally publishedYes


  • Autotaxin
  • Gynecologic tumor
  • Lysophosphatidic acid
  • Lysophosphatidylcholine
  • Lysophospholipase D
  • Peritoneal fluid
  • Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)


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