Periodontal gene transfer by ultrasound and nano/ microbubbles

R. Chen, M. Chiba, S. Mori, M. Fukumoto, T. Kodama

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

A non-viral gene delivery approach with nano/microbubbles and ultrasound offers opportunities for targeting soft tissues for gene therapy. The periodontium is a complex structure comprised of hard (cementum, alveolar bone) and soft tissues (periodontal ligament, gingivae). We hypothesized that our established gene delivery method would allow the periodontal tissue to be targeted for transfection for gene therapy. Expression kinetics and sites of transfection sites with this approach were investigated in rat periodontal tissue. Bioluminescence imaging revealed that transient gene expression was induced at day 1 post-transfection, while confocal microscopy showed that gene expression was localized in the muscle cells of gingival tissues. These findings indicate that regular transfection with this approach results in high gene expression, facilitating gene therapy for periodontal disease involving alveolar bone resorption.

Original languageEnglish
Pages (from-to)1008-1013
Number of pages6
JournalJournal of dental research
Volume88
Issue number11
DOIs
Publication statusPublished - 2009 Nov

Keywords

  • Gene therapy
  • Membrane permeability
  • Non-viral vector
  • Periodontal tissues
  • Ultrasound

ASJC Scopus subject areas

  • Dentistry(all)

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