Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells

Naoko Ohkama-Ohtsu, Sachiko Ichida, Hiroko Yamaya, Takuji Ohwada, Manabu Itakura, Yoshino Hara, Hisayuki Mitsui, Takakazu Kaneko, Satoshi Tabata, Kouhei Tejima, Kazuhiko Saeki, Hirofumi Omori, Makoto Hayashi, Takaki Maekawa, Yoshikatsu Murooka, Shigeyuki Tajima, Kenshiro Simomura, Mika Nomura, Toshiki Uchiumi, Akihiro SuzukiYoshikazu Shimoda, Mikiko Abe, Kiwamu Minamisawa, Yasuhiro Arima, Tadashi Yokoyama

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Abstract: In leguminous root nodules, rhizobia differentiate into morphology specific to symbiosis, called bacteroids. As bacteroids are surrounded with peribacteroid membranes filled with peribacteroid solution (PBS), it is considered that PBS contains substances inducing differentiation of rhizobia into bacteroids. In this study, genome-wide expression profiles of Bradyrhizobium japonicum cells cultured in PBS purified from root nodule of soybean (Glycine max L.) were compared with those of bacteroids using macroarray. PBS treatment preferentially induced regions in a large symbiosis island including various symbiosis relevant genes such as nod, fix, nol and noe, in which 75% of regions were commonly induced in bacteroids, while general repressions outside of the symbiosis island seen in bacteroids were not observed in PBS treated cells. The present results suggest that PBS contained some, but not all, substances inducing expression of the genes which are involved in differentiation into bacteroids.

    Original languageEnglish
    Pages (from-to)461-470
    Number of pages10
    JournalSoil Science and Plant Nutrition
    Volume61
    Issue number3
    DOIs
    Publication statusPublished - 2015 May 4

    Keywords

    • Bradyrhizobium japonicum
    • bacteroid
    • macroarray
    • peribacteroid solution
    • soybean

    ASJC Scopus subject areas

    • Soil Science
    • Plant Science

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