TY - JOUR
T1 - Perfusion using oxygenated buffer containing prostaglandin E 1 before cold preservation prevents warm ischemia-reperfusion injury in liver grafts from non-heart-beating donors
AU - Hara, Y.
AU - Akamatsu, Y.
AU - Kobayashi, Y.
AU - Iwane, T.
AU - Satomi, S.
PY - 2010/12
Y1 - 2010/12
N2 - We have previously reported that perfusion using warm oxygenated buffer before cold preservation (preperfusion) improved the viability of liver grafts from non-heart-beating donors. We demonstrated that adenosine triphosphate content was restored and apoptosis was reduced. The objective of the present study was to evaluate mitochondrial functions after this preperfusion and the effects of addition of prostaglandin E 1 (PGE 1) to the preperfusion buffer. Preperfusion improved portal flow, bile production, and mitochondrial function, and reduced alanine aminotransferase levels in the perfusate. Addition of PGE 1 significantly increased bile production and suppressed alanine aminotransferase and tumor necrosis factor-α levels. PGE 1 minimized mitochondrial membrane damage and ischemic injury after liver graft reperfusion. Release of mitochondrial cytochrome c was suppressed by addition of PGE 1. In conclusion, perfusion using oxygenated buffer containing PGE 1 before cold preservation significantly prevented cellular damage, protected mitochondrial function, and suppressed the release of mitochondrial cytochrome c in livers undergoing warm ischemia-reperfusion injury. This method shows promise for reducing cellular damage in non-heart-beating donor liver grafts.
AB - We have previously reported that perfusion using warm oxygenated buffer before cold preservation (preperfusion) improved the viability of liver grafts from non-heart-beating donors. We demonstrated that adenosine triphosphate content was restored and apoptosis was reduced. The objective of the present study was to evaluate mitochondrial functions after this preperfusion and the effects of addition of prostaglandin E 1 (PGE 1) to the preperfusion buffer. Preperfusion improved portal flow, bile production, and mitochondrial function, and reduced alanine aminotransferase levels in the perfusate. Addition of PGE 1 significantly increased bile production and suppressed alanine aminotransferase and tumor necrosis factor-α levels. PGE 1 minimized mitochondrial membrane damage and ischemic injury after liver graft reperfusion. Release of mitochondrial cytochrome c was suppressed by addition of PGE 1. In conclusion, perfusion using oxygenated buffer containing PGE 1 before cold preservation significantly prevented cellular damage, protected mitochondrial function, and suppressed the release of mitochondrial cytochrome c in livers undergoing warm ischemia-reperfusion injury. This method shows promise for reducing cellular damage in non-heart-beating donor liver grafts.
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U2 - 10.1016/j.transproceed.2010.09.085
DO - 10.1016/j.transproceed.2010.09.085
M3 - Article
C2 - 21168603
AN - SCOPUS:78650431915
VL - 42
SP - 3973
EP - 3976
JO - Transplantation Proceedings
JF - Transplantation Proceedings
SN - 0041-1345
IS - 10
ER -