Pentanoic acid induces thymic stromal lymphopoietin production through Gq/11 and Rho-associated protein kinase signaling pathway in keratinocytes

Natsumi Mizuno, Koudai Abe, Yukari Morishita, Saori Yamashita, Ryosuke Segawa, Jiangxu Dong, Takahiro Moriya, Masahiro Hiratsuka, Noriyasu Hirasawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Thymic stromal lymphopoietin (TSLP) plays an important role in allergic skin inflammation. Short-chain fatty acids (SCFAs), including pentanoic acid, are products of bacterial metabolism and are associated with allergic skin disorders. However, whether SCFAs induce TSLP production is still unclear. In this study, we evaluated the effect of SCFAs on TSLP production and found that pentanoic acid was the most efficacious of the tested SCFAs. The Gq/11 inhibitor YM-254890 and the Rho-associated protein kinase (ROCK) inhibitor Y-27632 inhibited pentanoic acid-induced TSLP production, as did transfection with Gq/11 siRNA. These results suggested that pentanoic acid-induced TSLP production was mediated by Gq/11 and ROCK, providing insights into a novel TSLP production pathway in keratinocytes. The novel mechanism of TSLP production is expected to support the development of TSLP-regulating approaches in allergic skin disorders.

Original languageEnglish
Pages (from-to)216-223
Number of pages8
JournalInternational Immunopharmacology
Volume50
DOIs
Publication statusPublished - 2017 Sep

Keywords

  • G
  • GPCR
  • Rho
  • Rho-associated protein kinase
  • Short chain fatty acid
  • TSLP

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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