Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium

Hiroyuki Imachi, Yuji Sekiguchi, Yoichi Kamagata, Satoshi Hanada, Akiyoshi Ohashi, Hideki Harada

Research output: Contribution to journalArticlepeer-review

154 Citations (Scopus)

Abstract

An anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium, strain SIT, isolated previously from granular sludge in a thermophilic upflow anaerobic sludge blanket (UASB) reactor, was characterized. The strain could grow fermentatively on pyruvate and fumarate in pure culture. The strain grew on propionate, ethanol, lactate, 1-butanol, 1-pentanol, 1,3-propanediol, 1-propanol and ethylene glycol in co-culture with the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus strain ΔHT . The optimum temperature for growth was 55 °C and the pH optimum was 7.0. The G+C content of the DNA was 52.8 mol %. Strain SIT contained MK-7 and MK-7(H4) as the major quinones and contained iso-C15:0 as the major fatty acid. Based on 16S rDNA sequence analysis, strain SIT formed a novel lineage within the Gram-positive, spore-forming, sulphate-reducing bacterial group Desulfotomaculum. However, the strain lacked the ability to conduct dissimilatory sulphate reduction. Instead, it could reduce fumarate to succinate with concomitant growth on several organic substances as electron donor. These phenotypic and genetic properties support the formation of a novel species of a new genus, for which the name Pelotomaculum thermopropionicum gen. nov., sp. nov. is proposed. The type strain is strain SIT (= DSM 13744T = JCM 10971T).

Original languageEnglish
Pages (from-to)1729-1735
Number of pages7
JournalInternational journal of systematic and evolutionary microbiology
Volume52
Issue number5
DOIs
Publication statusPublished - 2002 Sep 1

Keywords

  • Anaerobe
  • Pelotomaculum thermopropionicum gen. nov.
  • Propionate oxidation
  • Sp. nov
  • Syntroph
  • Thermophile

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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