Pelotomaculum propionicium sp. nov., an anaerobic, mesophilic, obligately syntrophic, propionate-oxidizing bacterium

Hiroyuki Imachi, Sanae Sakai, Akiyoshi Ohashi, Hideki Harada, Satoshi Hanada, Yoichi Kamagata, Yuji Sekiguchi

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

An anaerobic, mesophilic, syntrophic, propionate-oxidizing bacterium, strain MGPT, was isolated as a defined co-culture with Methanospirillum hungatei from the methanogenic sludge of a mesophilic upflow anaerobic sludge blanket (UASB) reactor. The strain grew in the presence of propionate, but only in co-culture with methanogens, suggesting that it is an obligately syntrophic bacterium. The optimum temperature for growth was 37 °C, and the optimum pH was between 6.5 and 7.2. Based on comparative 16S rRNA gene sequence analysis, strain MGPT was affiliated with subcluster Ih of 'Desulfotomaculum cluster I', in which it was found to be moderately related to known species of the genera Pelotomaculum and Cryptanaerobacter. Similar to known species of the genus Pelotomaculum, strain MGPT could degrade propionate in syntrophy, but had no ability to reduce sulfate, sulfite and thiosulfate. Further phenotypic and genetic studies supported the affiliation of the strain as a novel species in this genus, for which the name Pelotomaculum propionicicum sp. nov. is proposed. The type strain is MGPT (= DSM 15578T=JCM 11929T). The strain has been deposited in the DSM and JCM culture collections as a defined co-culture with Methanospirillum hungatei.

Original languageEnglish
Pages (from-to)1487-1492
Number of pages6
JournalInternational journal of systematic and evolutionary microbiology
Volume57
Issue number7
DOIs
Publication statusPublished - 2007 Jul

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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