Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family

Yuto Takenaka, Kohei Kato, Mari Ogawa-Ohnishi, Kana Tsuruhama, Hiroyuki Kajiura, Kenta Yagyu, Atsushi Takeda, Yoichi Takeda, Tadashi Kunieda, Ikuko Hara-Nishimura, Takeshi Kuroha, Kazuhiko Nishitani, Yoshikatsu Matsubayashi, Takeshi Ishimizu

Research output: Contribution to journalLetter

20 Citations (Scopus)

Abstract

Pectin is one of the three key cell wall polysaccharides in land plants and consists of three major structural domains: homogalacturonan, rhamnogalacturonan I (RG-I) and RG-II. Although the glycosyltransferase required for the synthesis of the homogalacturonan and RG-II backbone was identified a decade ago, those for the synthesis of the RG-I backbone, which consists of the repeating disaccharide unit [→2)-α-l-Rha-(1 → 4)-α-d-GalUA-(1→], have remained unknown. Here, we report the identification and characterization of Arabidopsis RG-I:rhamnosyltransferases (RRTs), which transfer the rhamnose residue from UDP-β-l-rhamnose to RG-I oligosaccharides. RRT1, which is one of the four Arabidopsis RRTs, is a single-spanning transmembrane protein, localized to the Golgi apparatus. RRT1 was highly expressed during formation of the seed coat mucilage, which is a specialized cell wall with abundant RG-I. Loss-of-function mutation in RRT1 caused a reduction in the level of RG-I in the seed coat mucilage. The RRTs belong to a novel glycosyltransferase family, now designated GT106. This is a large plant-specific family, and glycosyltransferases in this family seem to have plant-specific roles, such as biosynthesis of plant cell wall polysaccharides.

Original languageEnglish
Pages (from-to)669-676
Number of pages8
JournalNature Plants
Volume4
Issue number9
DOIs
Publication statusPublished - 2018 Sep 1

ASJC Scopus subject areas

  • Plant Science

Fingerprint Dive into the research topics of 'Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family'. Together they form a unique fingerprint.

  • Cite this

    Takenaka, Y., Kato, K., Ogawa-Ohnishi, M., Tsuruhama, K., Kajiura, H., Yagyu, K., Takeda, A., Takeda, Y., Kunieda, T., Hara-Nishimura, I., Kuroha, T., Nishitani, K., Matsubayashi, Y., & Ishimizu, T. (2018). Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family. Nature Plants, 4(9), 669-676. https://doi.org/10.1038/s41477-018-0217-7