PCR-RFLP analysis of cytomegalovirus infections associated with bone marrow transplantation in Japanese children

Satoshi Kawasaki, Hitoshi Oshitani, Hiroshi Suzuki, Masaaki Arakawa, Katsumi Mizuta, Masue Imaizumi, Shigeru Tsuchiya, Tasuke Konno

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In order to investigate the longitudinal molecular epidemiology of cytomegalovirus (CMV) infections associated with bone marrow transplantation (BMT) in Japanese children, we analyzed 36 CMV strains from 11 cases. Three regions (DNA polymerase, glycoprotein H, and immediate-early regions) of CMV DNA were amplified by polymerase chain reaction (PCR), and amplified products were each digested with two restriction enzymes, followed by electrophoresis. These restriction fragment length polymorphism (RFLP) analyses allowed the differentiation of 36 strains into 13 genotypes. Each patient excreted his or her own CMV with distinct genotype over the study period of up to one year. CMVs of two different genotypes were recovered during a one-month study from one recipient, who received a peripheral blood stem cell transplantation. Although the majority of patients and donors were CMV-seropositive before BMT, multiple CMV infections might not be common and the reactivation of latently infected CMV might be prominent in Japanese children receiving transplants.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume43
Issue number4
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • BMT
  • Cytomegalovirus
  • PCR-RFLP

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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