PCR-restriction fragment length polymorphism analysis of genes coding for 16S rRNA in Veillonella spp.

Takuichi Sato, Michiko Sato, Junko Matsuyama, Etsuro Hoshino

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the American Type Culture Collection type strains of the genus Veillonella, i.e., V. atypica, V. caviae, V. criceti, V. dispar, V. parvula, V. ratti, and V. rodentium. Whole-cell protein profiles were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for comparative purposes. The 16S rRNA genes were amplified by PCR, and RFLP analysis of the 16S rDNA was performed with MnlI and Sau3AI. MnlI produced six RFLP patterns for seven type strains, since the patterns for V. atypica and V. caviae were the same. RFLP patterns with Sau3AI could distinguish between V. atypica and V. caviae. The type strains of Veillonella species were easily distinguished by 16S rDNA PCR-RFLP.

Original languageEnglish
Pages (from-to)1268-1270
Number of pages3
JournalInternational Journal of Systematic Bacteriology
Volume47
Issue number4
DOIs
Publication statusPublished - 1997 Oct

ASJC Scopus subject areas

  • Microbiology
  • Immunology

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