PCR-based detection of blood parasites in cattle and adult Rhipicephalus appendiculatus ticks

Shinji Yamada, Satoru Konnai, Saiki Imamura, Martin Simuunza, Mwelwa Chembensofu, Amos Chota, Andrew Nambota, Misao Onuma, Kazuhiko Ohashi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


To ascertain the infection rate for tick-borne pathogens in Zambia, an epidemiological survey of Theileria parva, Babesia bigemina and Anaplasma marginale in traditionally managed Sanga cattle was conducted using PCR. Of the 71 native Zambian cattle, 28 (39.4%) were positive for T. parva, 16 (22.5%) for B. bigemina and 34 (47.9%) for A. marginale. The mixed infection rate in cattle was 8.5% (6/71), 16.9% (12/71), 7.0% (5/71) and 2.8% (2/71) for T. parva/B. bigemina, T. parva/A. marginale, B. bigemina/A. marginale and T. parva/B. bigemina/A. marginale, respectively. To predict the risk for transmission of tick-borne pathogens from ticks to cattle, a total of 74 Rhipicephalus appendiculatus ticks were collected from a location where cattle had been found positive for T. parva. Of the ticks collected, 10 (13.5%) were found to be PCR-positive for T. parva. The results suggest that the infection rate for tick-borne pathogens was relatively high in Sanga cattle and that adult R. appendiculatus ticks were highly infected with T. parva.

Original languageEnglish
Pages (from-to)352-355
Number of pages4
JournalVeterinary Journal
Issue number2
Publication statusPublished - 2009 Nov


  • Anaplasma marginale
  • Babesia bigemina
  • Cattle
  • Rhipicephalus appendiculatus
  • Theileria parva
  • Zambia

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)


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