PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast

Yasukazu Daigaku, Thomas J. Etheridge, Yuka Nakazawa, Mayumi Nakayama, Adam T. Watson, Izumi Miyabe, Tomoo Ogi, Mark A. Osborne, Antony M. Carr

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase δ with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling.

Original languageEnglish
Article numbere1006789
JournalPLoS Genetics
Volume13
Issue number5
DOIs
Publication statusPublished - 2017 May

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Fingerprint

Dive into the research topics of 'PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast'. Together they form a unique fingerprint.

Cite this