Pax6 is required for making specific subpopulations of granule and periglomerular neurons in the olfactory bulb

Minoree Kohwi, Noriko Osumi, John L.R. Rubenstein, Arturo Alvarez-Buylla

Research output: Contribution to journalArticlepeer-review

258 Citations (Scopus)

Abstract

The subventricular zone (SVZ) produces different subclasses of olfactory bulb (OB) interneurons throughout life. Little is known about the molecular mechanisms controlling the production of different types of interneurons. Here we show that most proliferating adult SVZ progenitors express the transcription factor Pax6, but only a small subpopulation of migrating neuroblasts and new OB interneurons derived from these progenitors retains Pax6 expression. To elucidate the cell-autonomous role of Pax6 in OB neurogenesis, we transplanted green fluorescent protein-expressing embryonic forebrain progenitors of the dorsal lateral ganglionic eminence from Pax6 mutant Small Eye (Pax6 Sey/Sey) mice into the SVZ of adult wild-type mice. Pax6 Sey/Sey progenitors produce neuroblasts capable of migrating into the OB but fail to generate dopaminergic periglomerular and superficial granule cells. Interestingly, superficial granule neurons also express mRNA for tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Our data show that SVZ neuroblasts are heterogeneous and that Pax6 is required in a cell-autonomous manner for the production of cells in the dopaminergic lineage.

Original languageEnglish
Pages (from-to)6997-7003
Number of pages7
JournalJournal of Neuroscience
Volume25
Issue number30
DOIs
Publication statusPublished - 2005 Jul 27

Keywords

  • Adult neurogenesis
  • Dopaminergic periglomerular cells
  • Interneurons
  • Mouse
  • Olfactory bulb
  • Sey

ASJC Scopus subject areas

  • Neuroscience(all)

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