Pax6 controls the expression of Lewis x epitope in the embryonic forebrain by regulating α1,3-Fucosyltransferase IX expression

Yasushi Shimoda, Youichi Tajima, Taka Osanai, Asao Katsume, Michinori Kohara, Takashi Kudo, Hisashi Narimatsu, Noriko Takashima, Yasuo Ishii, Shun Nakamura, Noriko Osumi, Yutaka Sanai

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Pax6 is a transcription factor involved in brain patterning and neurogenesis. Expression of Pax6 is specifically observed in the developing cerebral cortex, where Lewis x epitope that is thought to play important roles in cell interactions is colocalized. Here we examined whether Pax6 regulates localization of Lewis x using Pax6 mutant rat embryos. The Lewis x epitope disappeared in the Pax6 mutant cortex, and activity of α1,3-fucosyltransferase, which catalyzed the last step of Lewis x biosynthesis, drastically decreased in the mutant cortex as compared with the wild type. Furthermore, expression of a fucosyltransferase gene, FucT-IX, specifically decreased in the mutant, while no change was seen for expression of another fucosyltransferase gene, FucT-IV. These results strongly suggest that Pax6 controls Lewis x expression in the embryonic brain by regulating FucT-IX gene expression.

Original languageEnglish
Pages (from-to)2033-2039
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number3
DOIs
Publication statusPublished - 2002 Jan 18

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Shimoda, Y., Tajima, Y., Osanai, T., Katsume, A., Kohara, M., Kudo, T., Narimatsu, H., Takashima, N., Ishii, Y., Nakamura, S., Osumi, N., & Sanai, Y. (2002). Pax6 controls the expression of Lewis x epitope in the embryonic forebrain by regulating α1,3-Fucosyltransferase IX expression. Journal of Biological Chemistry, 277(3), 2033-2039. https://doi.org/10.1074/jbc.M108495200