Patterning of an ascidian embryo along the anterior-posterior axis through spatial regulation of competence and induction ability by maternally localized PEM

Gaku Kumano, Hiroki Nishida

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

In ascidian embryos, anterior-posterior (A-P) patterning of the vegetal cells is regulated by posteriorizing activities of a localized egg region known as posterior-vegetal cortex/cytoplasm (PVC). PEM is an essential component of the PVC and is involved in the posterior-specific cell cleavage pattern. Here we report a novel function of PEM independently of its function in cleavage regulation; it controls cell fate by excluding competence to respond to the FGF signal for notochord induction from posterior-vegetal cells. PEM was found to regulate the nuclear accumulation of β-catenin, an upstream activator of the competence factor. PEM also influences A-P patterning in the animal hemisphere. It was found to regulate FGF signal expression and restrict the occurrence of brain induction only in the anterior region. Our results suggest a model in which PEM patterns the embryo along the A-P axis through regulation of the spatial distribution of competence and induction ability.

Original languageEnglish
Pages (from-to)78-88
Number of pages11
JournalDevelopmental Biology
Volume331
Issue number1
DOIs
Publication statusPublished - 2009 Jul 1

Keywords

  • Anterior-posterior axis
  • Ascidian
  • Competence
  • FGF
  • Induction
  • PEM
  • β-catenin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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