Patterning adherent cells within microchannels by combination of electrochemical biolithography and dielectrophoresis

Research output: Contribution to conferencePaperpeer-review

Abstract

We report a method to create cell-adhesive regions and to position adherent cells on the newly created regions in sequence within a microfluidic channel. One of the microelectrodes fabricated at the channel wall was used for locally electrogenerating hypobromous acid that renders the opposite face of the channel protein-adsorptive. After the fibronectin was immobilized on the treated region, an ac voltage was applied to the microelectrodes array in the presence of suspended HeLa cells. Since a repulsive force of negative dielectrophoresis directs the cells toward the weakest region of the nonuniform electric field, the cells were positioned on the fibronectinpatterned region to allow the cell adhesion, even in the presence of fluid flow.

Original languageEnglish
Pages570-572
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Keywords

  • Biolithography
  • Cell adhesion
  • Dielectrophoresis
  • Microfluidic device

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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