Patterning adherent cells within microchannels by combination of electrochemical biolithography technique and repulsive dielectrophoretic force

Hirokazu Kaji, Masahiko Hashimoto, Soichiro Sekine, Takeaki Kawashima, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We describe a method to create cell-adhesive regions and to position adherent cells on the newly created regions in sequence within a microfluidic channel. One of the microelectrodes fabricated at the channel wall was used for locally electrogenerating hypobromous acid that renders the opposite face of the channel protein-adsorptive. After the fibronectin was immobilized on the treated region, an ac voltage (1 MHz, 20 Vpp) was applied to the microelectrodes array in the presence of suspended HeLa cells. Since a repulsive force of negative dielectrophoresis (DEP) directs the cells toward the weakest region of the nonuniform electric field, the cells were positioned on the fibronectin-patterned region to allow the cell adhesion, even in the presence of fluid flow (< 0.1 μL min-1). By repeating the above process, two types of cells could be patterned in the microchannel.

Original languageEnglish
Pages (from-to)555-558
Number of pages4
JournalElectrochemistry
Volume76
Issue number8
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Cell adhesion
  • Dielectrophoresis
  • Electrochemical biolithography
  • Microfluidic device

ASJC Scopus subject areas

  • Electrochemistry

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