Pattern formation and molecular transport of histidine-tagged GFPs using supported lipid bilayers

Hiroshi Nakashima, Kazuaki Furukawa, Yoshiaki Kashimura, Koji Sumitomo, Youichi Shinozaki, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We fabricated a heterogeneous supported lipid bilayer (SLB) by employing binary lipid mixtures comprising a saturated acyl chain DSPC and an unsaturated acyl chain nickel-chelating lipid. By using the specific adsorption properties of histidine-tagged proteins (His-tagged GFPs) in relation to nickel-chelating lipids, we demonstrated protein pattern formation on the SLB corresponding to the phase separation pattern of the SLB. In addition, by using a lipid mixture consisting of an unsaturated acyl chain DOPC and a nickel-chelating lipid, and His-tagged GFPs, we succeeded in transporting the proteins along a hydrophilic micropattern on a SiO2 substrate. The protein transport is induced by the self-spreading behavior of a fluid SLB with a kinetic spreading coefficient β = 10.4 μm2 s-1. This method provides a guide for strategically carrying various biomolecules to specific positions by using a soft biointerface on a solid surface. In addition, the results demonstrate the importance of using techniques that allow the controlled manipulation of biomolecules based on the static or dynamic properties of the SLB platform.

Original languageEnglish
Pages (from-to)12716-12721
Number of pages6
JournalLangmuir
Volume26
Issue number15
DOIs
Publication statusPublished - 2010 Aug 3
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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