TY - JOUR
T1 - Pathological features of Cryptosporidium andersoni-induced lesions in SCID mice
AU - Masuno, Koichi
AU - Yanai, Tokuma
AU - Sakai, Hiroki
AU - Satoh, Masaaki
AU - kai, Chieko
AU - Nakai, Yutaka
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/7
Y1 - 2013/7
N2 - To assess the infectivity and the istopathological features of Cryptosporidium andersoni (. C. andersoni) in laboratory animals, SCID mice were orally inoculated with oocysts of C. andersoni. Starting one week after inoculation, the SCID mice began shedding oocysts, and this continued for ten weeks. Histopathologically, myriads of C. andersoni were observed on the apical surface of the epithelium in the gastric pit of the glandular stomach. There were few lesions in the gastric epithelium except C. andersoni adhesion. In the lamina propria of the affected mucosa, minimum infiltration of inflammatory cells was observed. Immunohistochemically, C. andersoni demonstrated a positive reaction to a number of primary antibodies of Cryptosporidium parvum. In the experiment described here, few increases were seen in apoptotic epithelial cells in the affected mucosas of the SCID mice, and the nuclear augmentation was not enhanced. It was hypothesized that the absence of apoptosis and cell division were due to a lack of inflammatory cell reaction in the lamina propria.
AB - To assess the infectivity and the istopathological features of Cryptosporidium andersoni (. C. andersoni) in laboratory animals, SCID mice were orally inoculated with oocysts of C. andersoni. Starting one week after inoculation, the SCID mice began shedding oocysts, and this continued for ten weeks. Histopathologically, myriads of C. andersoni were observed on the apical surface of the epithelium in the gastric pit of the glandular stomach. There were few lesions in the gastric epithelium except C. andersoni adhesion. In the lamina propria of the affected mucosa, minimum infiltration of inflammatory cells was observed. Immunohistochemically, C. andersoni demonstrated a positive reaction to a number of primary antibodies of Cryptosporidium parvum. In the experiment described here, few increases were seen in apoptotic epithelial cells in the affected mucosas of the SCID mice, and the nuclear augmentation was not enhanced. It was hypothesized that the absence of apoptosis and cell division were due to a lack of inflammatory cell reaction in the lamina propria.
KW - Alimentary tract
KW - Cryptosporidium
KW - Pathology
KW - SCID mouse
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U2 - 10.1016/j.exppara.2013.02.006
DO - 10.1016/j.exppara.2013.02.006
M3 - Article
C2 - 23499882
AN - SCOPUS:84877906768
VL - 134
SP - 381
EP - 383
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 3
ER -