Pathological alterations of chondroitin sulfate moiety in postmortem hippocampus of patients with schizophrenia

Takayuki Yukawa, Yuriko Iwakura, Nobuyuki Takei, Mami Saito, Yuichiro Watanabe, Kazuhiko Toyooka, Michihiro Igarashi, Kazuhiro Niizato, Kenichi Oshima, Yasuto Kunii, Hirooki Yabe, Junya Matsumoto, Akira Wada, Mizuki Hino, Shuji Iritani, Shin ichi Niwa, Ryoko Takeuchi, Hitoshi Takahashi, Akiyoshi Kakita, Toshiyuki SomeyaHiroyuki Nawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Perineuronal nets comprise chondroitin sulfate moieties and their core proteins, and their neuropathological alterations have been implicated in schizophrenia. To explore the molecular mechanism of the perineuronal net impairments in schizophrenia, we measured the immunoreactivity of chondroitin sulfate moieties, major components of perineuronal nets, in three brain regions (postmortem dorsolateral prefrontal cortex, caudate nucleus, and hippocampus) of schizophrenia patients and control subjects. Immunoblotting for chondroitin 4-sulfate and chondroitin 6-sulfate moieties revealed a significant increase in intensity of a 180 kD band of chondroitin 4-sulfate immunoreactivity in the hippocampus of patients, although we detected no significant alteration in their immunoreactivities with any other molecular sizes or in other brain regions. The levels of immunoreactivity were not correlated with postmortem interval, age, or storage time. We failed to find such an increase in a similar molecular range of the chondroitin 4-sulfate immunoreactivity in the hippocampus of the rats chronically treated with haloperidol. These results suggest that the level alteration of the chondroitin 4-sulfate moiety might contribute to the perineuronal net abnormality found in patients with schizophrenia.

Original languageEnglish
Pages (from-to)940-946
Number of pages7
JournalPsychiatry Research
Volume270
DOIs
Publication statusPublished - 2018 Dec
Externally publishedYes

Keywords

  • Haloperidol
  • Hippocampus
  • Perineuronal net
  • Postmortem brain

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry

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