Partitioning of the matrix fraction of the golgi apparatus during mitosis in animal cells

Joachim Seemann, Marc Pypaert, Tomohiko Taguchi, Jörg Malsam, Graham Warren

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The Golgi apparatus is partitioned during mitosis in animal cells by a process of fragmentation, dispersal, and reassembly in each daughter cell. We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein. After these treatments, Golgi enzymes moved back to the endoplasmic reticulum, leaving behind a matrix of Golgi structural proteins. Under these conditions, cells still entered and exited mitosis normally, and their Golgi matrix partitioned in a manner very similar to that of the complete organelle. Thus, the matrix may be the partitioning unit of the Golgi apparatus and may carry the Golgi enzyme-containing membranes into the daughter cells.

Original languageEnglish
Pages (from-to)848-851
Number of pages4
JournalScience
Volume295
Issue number5556
DOIs
Publication statusPublished - 2002 Feb 1
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Partitioning of the matrix fraction of the golgi apparatus during mitosis in animal cells'. Together they form a unique fingerprint.

  • Cite this