TY - JOUR
T1 - Participation of hemocyte proteinase in dissociation of the fat body on pupation of Sarcophaga peregrina (flesh fly)
AU - Kurata, Shoichiro
AU - Saito, Hiromi
AU - Natori, Shunji
PY - 1990/1/1
Y1 - 1990/1/1
N2 - In holometabolous insects, larval tissues are degraded on pupation. Previously, we established an in vitro system in which Sarcophaga fat body dissociates in the presence of pupal hemocytes, mimicking the situation in vivo (Kutata et al., J. Insect Physiol. 35, 559-565, 1989). In this paper, we showed that chymostatin and phenylmethylsulfonyl fluoride prevented dissociation of fat body in this system. Moreover, of the various proteinases tested, only chymotrypsin degraded the fat body in vitro. These results suggested that a chymotrypsin-like proteinase of hemocytes participates in dissociation of the fat body at the early pupal stage.
AB - In holometabolous insects, larval tissues are degraded on pupation. Previously, we established an in vitro system in which Sarcophaga fat body dissociates in the presence of pupal hemocytes, mimicking the situation in vivo (Kutata et al., J. Insect Physiol. 35, 559-565, 1989). In this paper, we showed that chymostatin and phenylmethylsulfonyl fluoride prevented dissociation of fat body in this system. Moreover, of the various proteinases tested, only chymotrypsin degraded the fat body in vitro. These results suggested that a chymotrypsin-like proteinase of hemocytes participates in dissociation of the fat body at the early pupal stage.
KW - chymostatin
KW - dissociation
KW - fat body
KW - hemocytes
KW - proteinase
KW - Sarcophaga peregrina
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U2 - 10.1016/0020-1790(90)90027-R
DO - 10.1016/0020-1790(90)90027-R
M3 - Article
AN - SCOPUS:0001650304
VL - 20
SP - 461
EP - 465
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
SN - 0965-1748
IS - 5
ER -