Intraglomerular expression of complement receptors (CR) was investigated chronologically in 22 repeatedly biopsied patients with membranoproliferative glomerulonephritis (MPGN) type I by indirect immunoperoxidase staining using MoAbs. Patients were divided into two groups based on whether intraglomerular C3c deposition was decreased at the second biopsy (2nd Ex) (group A, n = 12), or not (group B, n = 10). At the first biopsy (1st Bx), the severity of glomerular injury and the degree of glomerular C3c deposition were compatible between the two groups. Four patterns of CR1 (CD35) expression on podocytes were recognized: normal; generally decreased; focally/segmentally lost; and completely lost. The numbers of CR3 (CD11b/CD18)- and CR4 (CD11c/CD18)-positive cells per glomerular cross-section were counted. At the 1st Ex, no significant difference was found in the number of CR3+ or CR4+ cells between the two groups. At the 2nd Ex, the numbers of both the CR3+ and CR4+ cells were significantly decreased only in group A (P < 0.01). The numbers of CR3+ and CR4+ cells were significantly higher in cases with moderate or marked C3c deposits than in those with no or mild C3c deposits. The intensity of CRI expression in group B was less than that in group A at both the 1st and 2nd Ex (1st, P < 0.05; 2nd, P < 0.01), and chronological improvement of CRI expression was observed only in group A. The severity of glomerular injury was increased only in group B (P< 0.01), and was associated with persistent massive proteinuria and hypocomplementaemia. Our results suggest that, in cases with an adverse outcome, a more severe defect of CRI initially exists and the expression of CR1 is not recoverable chronologically. This irreversible decrease or loss of CRI may partly contribute to the continuous C3c deposition and intraglomerular infiltration of CR3+ and CR4+ cells.
|Number of pages||8|
|Journal||Clinical and Experimental Immunology|
|Publication status||Published - 1995 May 24|
- Membranoproliferative glomerulonephritis type I
ASJC Scopus subject areas
- Immunology and Allergy