Participation of a cytochrome b5-like hemoprotein of outer mitochondrial membrane (OM cytochrome b) in NADH-semidehydroascorbic acid reductase activity of rat liver

Akio Ito, Shin ichi Hayashi, Touho Yoshida

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The participation of a cytochrome b5-like hemoprotein of outer mitochondrial membrane (OM cytochrome b) in the NADH-semidehydroascorbate (SDA) reductase activity of rat liver was studied. NADH-SDA reductase activity was strongly inhibited by antibodies against OM cytochrome b and NADH-cytochrome b5 reductase, whereas no inhibition was caused by anti-cytochrome b5 antibody. NADH-SDA reductase exhibited the same distribution pattern as OM cytochrome b-mediated rotenone-insensitive NADH-cytochrome c reductase activity among various subcellular fractions and submitochondrial fractions. Both activities were localized in outer mitochondrial membrane. These observations suggest that OM cytochrome b-mediated rotenone-insensitive NADH-cytochrome c reductase system participates in the NADH-SDA reductase activity of rat liver.

Original languageEnglish
Pages (from-to)591-598
Number of pages8
JournalBiochemical and biophysical research communications
Volume101
Issue number2
DOIs
Publication statusPublished - 1981 Jul 30
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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