TY - JOUR
T1 - Participation of a 200-kDa hemocyte membrane protein in the dissociation of the fat body at the metamorphosis of Sarcophaga
AU - Kurata, Shoichiro
AU - Kobayashi, Hideaki
AU - Natori, Shunji
N1 - Funding Information:
This study was supported by a Grant-in-Aid from the Ministry of Education, Science and
PY - 1991/7
Y1 - 1991/7
N2 - Three monoclonal antibodies were raised against a 200-kDa protein specifically expressed on the surface of hemocytes when Sacrophaga larvae pupated. One of these antibodies significantly inhibited dissociation of the fat body in the presence of pupal hemocytes, indicating that the 200-kDa protein is essential for dissociation of the fat body by the hemocytes. When the hemocytes were treated with a mixture of the monoclonal antibodies, they were found to secrete a significant amount of chymotrypsin-like proteinase. Moreover, the number of hemocytes expressing the 200-kDa protein increased significantly after puparium formation. These results suggested that some signal is transferred to the hemocytes via the 200-kDa protein when they interact with the basement membrane of the fat body and that this induces their secretion of a chymotrypsin-like proteinase essential for decomposition of the fat body.
AB - Three monoclonal antibodies were raised against a 200-kDa protein specifically expressed on the surface of hemocytes when Sacrophaga larvae pupated. One of these antibodies significantly inhibited dissociation of the fat body in the presence of pupal hemocytes, indicating that the 200-kDa protein is essential for dissociation of the fat body by the hemocytes. When the hemocytes were treated with a mixture of the monoclonal antibodies, they were found to secrete a significant amount of chymotrypsin-like proteinase. Moreover, the number of hemocytes expressing the 200-kDa protein increased significantly after puparium formation. These results suggested that some signal is transferred to the hemocytes via the 200-kDa protein when they interact with the basement membrane of the fat body and that this induces their secretion of a chymotrypsin-like proteinase essential for decomposition of the fat body.
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U2 - 10.1016/0012-1606(91)90458-F
DO - 10.1016/0012-1606(91)90458-F
M3 - Article
C2 - 2060701
AN - SCOPUS:0025730806
VL - 146
SP - 179
EP - 185
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -