Partial transfection of cells using laminar flows in microchannels

Lei Li, Yong Nie, Xuetao Shi, Hongkai Wu, Datian Ye, Hongda Chen

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

This manuscript describes a convenient method for partial transfection using a Y-shaped microchannel polydimethylsiloxane (PDMS)-glass chip and on-chip cationic lipid-mediated transfection. Enhanced green fluorescent protein genes (pEGFP-N2) were introduced into the COS-7 cells cultured in half of the channel, while red fluorescent protein genes (pDsRed-N1) were introduced into the cells cultured in another half of the channel. This on-chip partial transfection technique provides an avenue for the spatial control of transfection. It is possible to use this technique to perform parallel transfection on chips in order to study cell behaviors under two or more gene transfections in the same culture.

Original languageEnglish
Article number036503
JournalBiomicrofluidics
Volume5
Issue number3
DOIs
Publication statusPublished - 2011 Sep 20
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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