Partial reconstitution of the photosynthetic oxygen evolution system by rebinding of the 33-kDa polypeptide

Mitsue Miyao, Norio Murata

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Treatment with 2.6 M urea of the Photosystem II particles depleted of two polypeptides of 24 kDa and 18 kDa completely released a polypeptide of 33 kDa and eliminated the oxygen-evolution activity. The 33-kDa polypeptide rebound to the urea-treated particles and partially reactivated the oxygen evolution. A quantitative analysis of the rebinding suggests tha there is a specific binding site for the 33-kDa polypeptide on the membrane surface.

Original languageEnglish
Pages (from-to)375-378
Number of pages4
JournalFEBS Letters
Volume164
Issue number2
DOIs
Publication statusPublished - 1983 Dec 12
Externally publishedYes

Keywords

  • (Spinach chloroplast)
  • 33-kDa polypeptide
  • Oxygen evolution
  • Photosynthesis
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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