TY - JOUR
T1 - Partial purification and properties of glutathione peroxidase from carp hepatopancreas
AU - Nakano, T.
AU - Sato, M.
AU - Takeuchi, M.
PY - 1992/5
Y1 - 1992/5
N2 - 1. 1. Two glutathione peroxidases (GSH-Px), D1 and D2, were partially purified from the hepatopancreas of common carp Cyprinus carpio by ion-exchange, gel filtration, and hydrophobic chromatography. The enzymatic properties of those enzymes were examined. 2. 2. The D1 and D2 differed in following enzymatic properties. The pH optima of D1 and D2 were 8.0 and 9.0, respectively. 3. 3. When stored at pH 4.0 for 36 hr, D1 kept 70% of maximal activity while only 30% of that was shown in D2. 4. 4. D1 and D2 lost activity by 50% when incubated at 55°C and 65°C for 10 min, respectively.
AB - 1. 1. Two glutathione peroxidases (GSH-Px), D1 and D2, were partially purified from the hepatopancreas of common carp Cyprinus carpio by ion-exchange, gel filtration, and hydrophobic chromatography. The enzymatic properties of those enzymes were examined. 2. 2. The D1 and D2 differed in following enzymatic properties. The pH optima of D1 and D2 were 8.0 and 9.0, respectively. 3. 3. When stored at pH 4.0 for 36 hr, D1 kept 70% of maximal activity while only 30% of that was shown in D2. 4. 4. D1 and D2 lost activity by 50% when incubated at 55°C and 65°C for 10 min, respectively.
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U2 - 10.1016/0305-0491(92)90268-V
DO - 10.1016/0305-0491(92)90268-V
M3 - Article
C2 - 1526130
AN - SCOPUS:0026562438
VL - 102
SP - 31
EP - 35
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 1
ER -