Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia

Shintaro Iwashita, Takahiro Nobukuni, Shoji Tanaka, Mariko Kobayashi, Toshihiko Iwanaga, Hidetoshi B. Tamate, Tohru Masui, Ichiro Takahashi, Katsuyuki Hashimoto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region derived from part of a long interspersed DNA sequence element (LINE) in Ruminantia. However, the unique portion is completely missing in human and mouse BCNTs. Since no significant information on their function has been obtained by homology search, we at first examined cellular localization and biochemical characteristics of bovine BCNT to get a hint on its function. Subcellular fractionation and immunohistochemical analyses using a normal bovine epithelial cell line and bovine brain revealed that a significant amount of bovine BCNT is localized in the nuclei, while the major portion is present in the cytosol. Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These results show that BCNTs consist of a unique family, probably a substrate of casein kinase II, which may contribute further to the understanding of gene evolution. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)408-416
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1427
Issue number3
DOIs
Publication statusPublished - 1999 May 24
Externally publishedYes

Keywords

  • LINE insertion
  • Nuclear localization
  • Phosphoprotein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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