Partial male sterility in transgenic tobacco carrying antisense and sense PAL cDNA under the control of a tapetum-specific promoter

Narumi Matsuda, Tohru Tsuchiya, Sachie Kishitani, Yoshiyuki Tanaka, Kinya Toriyama

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) cataIyzes the first step in the synthesis of phenylpropanoids. An antisense or sense PAL cDNA of sweet potato under the control of a rapetum-specific promoter of rice was introduced into tobacco. A reduction in pollea fertility was observed in two out of seventeen antisense PAL transformants and in six out of nineteen sense PAL transformants. The pollen fertility of these plants ranged from 8% to 60%. The distorted pollen grains that did not germinate lacked starch and flavonols. PAL activity in anthers at the microspore stage was also reduced to some extent and the level of PAL activity was positively correlated with the number of fertile pollen grains at the flowering stagre. Although it was uncleat how the antisense or sense transgene affected the PAL actuiiv in anthers, our results clearly demonstrate that the PAL activity in the anther tapetum has a significant effect on the developmen of microspores.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalPlant and Cell Physiology
Volume37
Issue number2
DOIs
Publication statusPublished - 1996 Mar

Keywords

  • Antisense inhibition
  • Male sterility
  • Phenyl-alanine ammonia-lyase (EC 4.3.1.5)
  • Pollen development
  • Tapetum-specific promoter
  • Transgenic tobacco

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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