TY - GEN
T1 - Parametric investigations for frequency-domain lifetime psp technique (FLIM)
AU - Sato, Hitomi
AU - Yorita, Daisuke
AU - Hilfer, Michael
AU - Henne, Ulrich
AU - Klein, Christian
AU - Saito, Yuji
AU - Nonomura, Taku
AU - Asai, Keisuke
N1 - Funding Information:
The authors are very grateful to Dr. R. Franke of PCO AG for his kind supports operating the pco.flim camera. We also would like to thank Dr. R. Brakman and J. L. Gonzalez Robles from DLR, AT-TUR for their support with the IR-camera.
Publisher Copyright:
© 2020, American Institute of Aeronautics and Astronautics Inc, AIAA. All rights reserved.
PY - 2020
Y1 - 2020
N2 - The effects of measurement parameters on frequency-domain fluorescence lifetime imaging (FLIM) with a new CMOS camera sensor (pco.flim) were investigated. The phase and demodulation indices were calculated from acquired images, and a function for the pressure is constructed. Pressure calibration tests performed with changing measurement parameters illustrate that increasing excitation modulation frequency significantly increases the pressure sensitivity but has a negative effect on a signal-to-noise ratio. The excitation modulation amplitude and its offset should be as high as possible for the reduction of the shot-noise influence. The pressure error calculated from the normalized phase index was the smallest at the modulation frequency of 20 kHz, while that from normalized demodulation index decreased as the modulation frequency increased. An impinging jet test has been conducted and the applicability of FLIM-PSP technique was investigated. Pressure distribution obtained from the test at the modulation frequencies of 20 kHz and 80 kHz were successfully compared to the pressure tap measurements. Considering the pressure error evaluation, the best results using the phase and demodulation indices were obtained at the modulation frequencies of 20 kHz and 80 kHz, respectively.
AB - The effects of measurement parameters on frequency-domain fluorescence lifetime imaging (FLIM) with a new CMOS camera sensor (pco.flim) were investigated. The phase and demodulation indices were calculated from acquired images, and a function for the pressure is constructed. Pressure calibration tests performed with changing measurement parameters illustrate that increasing excitation modulation frequency significantly increases the pressure sensitivity but has a negative effect on a signal-to-noise ratio. The excitation modulation amplitude and its offset should be as high as possible for the reduction of the shot-noise influence. The pressure error calculated from the normalized phase index was the smallest at the modulation frequency of 20 kHz, while that from normalized demodulation index decreased as the modulation frequency increased. An impinging jet test has been conducted and the applicability of FLIM-PSP technique was investigated. Pressure distribution obtained from the test at the modulation frequencies of 20 kHz and 80 kHz were successfully compared to the pressure tap measurements. Considering the pressure error evaluation, the best results using the phase and demodulation indices were obtained at the modulation frequencies of 20 kHz and 80 kHz, respectively.
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U2 - 10.2514/6.2020-0293
DO - 10.2514/6.2020-0293
M3 - Conference contribution
AN - SCOPUS:85092421642
SN - 9781624105951
T3 - AIAA Scitech 2020 Forum
BT - AIAA Scitech 2020 Forum
PB - American Institute of Aeronautics and Astronautics Inc, AIAA
T2 - AIAA Scitech Forum, 2020
Y2 - 6 January 2020 through 10 January 2020
ER -