Paramagnetic relaxation-based 19F MRI probe to detect protease activity

Shin Mizukami, Rika Takikawa, Fuminori Sugihara, Yuichiro Hori, Hidehito Tochio, Markus Wälchli, Masahiro Shirakawa, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

178 Citations (Scopus)

Abstract

A novel design principle for 19F MRI probes detecting protease activity was developed. This principle is based on 19F MRI signal quenching by the intramolecular paramagnetic effect from Gd3+. The intramolecular Gd3+ dramatically attenuated the 19F probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using 19F MRI.

Original languageEnglish
Pages (from-to)794-795
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number3
DOIs
Publication statusPublished - 2008 Jan 23
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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